COMPARATIVE ASSESSMENT OF BIOCHEMICAL AND IMMUNOLOGICAL MARKERS IN TUBERCULOSIS

Authors

  • Abhijit Ninghot Assistant Professor, Department of Biochemistry, Government Medical College, Nagpur.
  • Kanchan Mohod Associate Professor, Department of Biochemistry, MGIMS, Sevagram, Wardha.
  • Mohd. Sameer Khan Deputy Medical Superintendant, Nehariya Dispensary, Pench Area, Western Coalfield Ltd.
  • Azmat Kamal Ansari Demonstrator, Department of Biochemistry, Government Medical College, Chhindwara.
  • Satish Kumar Professor& Head, Department of Biochemistry, MGIMS, Sevagram, Wardha.

DOI:

https://doi.org/10.32553/ijmbs.v3i10.611

Keywords:

Adenosine deaminase, Lactate dehydrogenase, uberculosis antigen-antibody

Abstract

Introduction: Pulmonary tuberculosis is major public health problem in developing countries like India.Millions of people have died from tuberculosis. Many times it is difficult to get sputum sample from the patients. Some tests lack specificity, some other lack sensitivity. Hence, there is need of precise and faster diagnosis for patients attending hospitals. In this study, we compared the detection potential of biochemical and immunological markers(ADA, LDH and Mycobacterium tuberculosis H37Ra ES-31 & EST-6 antigens & antibodies based ELISAs) in pulmonary tuberculosis.

Methods: 50 pulmonary tuberculosis cases confirmed by sputum examinationfor acid fast bacilli (AFB) and 50 age and sex matched control subjects were included in this study.ADA & LDH were estimated by using commercial kits. Tubercular antigens and antibodies were detected by ELISA method.

Results: SerumADA detected pulmonary tuberculosis with sensitivityand specificityof 94%. Sensitivity and specificity of serum LDH in detecting pulmonary tuberculosis was found to be 94% and 36% respectively.Serumtubercular antigens detected pulmonary tuberculosis with sensitivity and specificity of 88%. Sensitivity and specificity of serum tubercular antibodies in detecting pulmonary tuberculosis was found to be 80% and 90% respectively.

Conclusion: Adenosine deaminase has better detection potential over other markers in pulmonary tuberculosis.

Keywords: Adenosine deaminase, Lactate dehydrogenase, Tuberculosis antigen-antibody

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Published

2019-10-16

How to Cite

Ninghot, A., Mohod, K., Khan, M. S., Ansari, A. K., & Kumar, S. (2019). COMPARATIVE ASSESSMENT OF BIOCHEMICAL AND IMMUNOLOGICAL MARKERS IN TUBERCULOSIS. International Journal of Medical and Biomedical Studies, 3(10), 80-85. https://doi.org/10.32553/ijmbs.v3i10.611

Issue

Vol. 3 No. 10 (2019)

Section

Research Articles

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