TO EXPLORE AND COMPARE BIOENHANCING ACTIVITY OF PIPERINE USING DIFFERENT ROUTES OF ADMINISTRATION (ORAL AND INTRAPERITONEAL)

Abstract

Background & Method: The present study was conducted at Dept. of Pharmacology, Sri Aurobindo Institute of Medical Sciences & Research Centre, Indore. Piperine and was used to isolate the pure alkaloid, piperine, the extract was dissolved in 5 ml of ethyl alcohol. To this solution, 10 ml of 10% w/v of alcoholic potassium hydroxide was added with constant stirring. It was then filtered and allowed to stand overnight. The yellow needle shaped crystals of piperine were separated the next day.

Result: Pentazocine given in combination with piperine intraperitoneally showed significantly greater increase in reaction time as compared to pentazocine given in combination with piperine orally at 4 hr (p < 0.05).Sertraline given in combination with piperine intraperitoneally showed slightly greater increase in immobility period (58%) as compared to sertraline given in combination with piperine orally (51%), although not significant (p > 0.05). The oral combination of sertraline and piperine had significantly better activity than even the sertraline i.p. group (p < 0.05).

Conclusion: Studies are recommended on such combinations which may prove to be very good complimentary regimens for the treatment of many disorders. Pharmacokinetic studies are also required to further explore the bioenhancing property and characterise exact extent and mechanisms of such interactions.

Keywords: Piperine, Pentazocine, Sertraline & Albino Mice.

Study Designed: Observational Study.