FERTILITY ENHANCING EFFECT OF ETHANOL EXTRACT OF Carica papaya L. SEED IN MICE

Abstract

ABSTRACT: This paper reports the fertility effects of the ethanol extract of  C. papaya L. seeds for the evaluation of determining the estrous cycle which plays an important role in increasing fertility success. This study aims to assess the length of the estrous cycle time through vaginal cytology images. The papaya seed extract dosages used consisted of: the control group (C) was given papaya seed extract 0 mg/40 g BW in 0.4 ml aquabidest ; treatment 1 (T1) was given papaya seed extract 2 mg/40 g BW in 0.4 ml aquabidest; treatment 2  (T2)  was  given papaya seed extract 4 mg/40 g  BW  in  0.4  ml  aquabidest; Treatment 3 (T3) was given papaya seed extract 8 mg/40 g BW 0.4 ml aquabidest. Samples from vaginal cytology were collected every 4 hours for three months, and stained with Giemsa and the data obtained were tabulated and analyzed descriptively. The results showed that giving papaya seed extract to female mice could prolong the estrous time for the pro-estrous phase and the estrous phase at a dose of 2 mg/40 g BW, 4 mg/40 g BW, and 8 mg/40 g BW. In the proestrous phase, the longest mean duration of the proestrous phase occurred in the 8 mg/40 g BW treatment (25 hours) and in the estrous phase the longest average duration of the estrous phase occurred in the 8 mg/40 g BW treatment. (80.4 hours), compared to controls. Meanwhile, papaya seed extract did not show any changes in the composition and structure of the epithelial cells of the vaginal swab. The conclusion of this study is that the estrous cycle time of mice can be determined by the method of vaginal cytology analysis.

KEYWORDS: Papaya seed extract, estrous cycle, vaginal cytology, female mice.